Electron Transfer Dissociation of Peptides Generated by Microwave D-Cleavage Digestion of Proteins
نویسندگان
چکیده
منابع مشابه
Electron transfer dissociation of peptides generated by microwave D-cleavage digestion of proteins.
The nonenzymatic digestion of proteins by microwave D-cleavage is an effective technique for site-specific cleavage at aspartic acid (D). This specific cleavage C-terminal to D residues leads to inherently large peptides (15-25 amino acids) that are usually relatively highly charged (above +3) when ionized by electrospray ionization (ESI) due to the presence of several basic amino acids within ...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2008
ISSN: 1535-3893,1535-3907
DOI: 10.1021/pr700671z